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Our mission is to provide UAMS investigators with convenient access to the latest technologies for genomic interrogation using Next Generation Sequencing (NGS), microarray, and ddPCR analysis.
The complete NGS service includes:
Instrumentation
Illumina Sequencers
Illumina Bead Array
Nucleic Acid extraction
NGS Library Preparation
QC for Nucleic Acids and NGS Libraries
Droplet Digital PCR (ddPCR)
DNA Fragment Size Selection
Leadership
Donald J. Johann, MSc, MD
Director
DJJohann@uams.edu
| Hours | Location |
|
|
University of Arkansas for Medical Sciences 4301 W. Markham St., slot # 753 Little Rock, AR 72205
|
UAMS CUSTOMER TERMS AND CONDITIONS FOR RESEARCH CORE FACILITIES SERVICES.
| Name | Role | Phone | Location | |
|---|---|---|---|---|
| Donald Johann |
Scientific Director
|
DJJohann@uams.edu
|
||
| Ping Li |
Operations Manager
|
(501) 526-6000 x 24316
|
PLi@uams.edu
|
WPRCI 324
|
| Shijie Liu |
Technologist
|
SLiu2@uams.edu
|
.
|
| Service list |
| ► Nucleic Acid / Library handling (5) | |||
| Name | Description | Price | |
|---|---|---|---|
| Nucleic Acid Extraction (per sample) |
Extraction of DNA and/or RNA from a variety of sample sources |
Internal
$16.34
Sample
|
|
| Nucleic acid QC (per 11 samples) |
Fluorometric nucliec acid quantification with Qubit & capillary electrophoresis with Fragment Analyzer |
Internal
$131.30
each
|
|
| Fragment isolation with PippinHT (per sample) |
Removal or isolation of specific nuclic acid or library fragments |
Internal
$28.77
Sample
|
|
| Library QC (per 11 samples) |
Fluorometric nucliec acid quantification with Qubit, capillary electrophoresis with Fragment Analyzer, & qPCR for functional validation of libraries |
Internal
$364.98
Sample
|
|
| Pooling fee (per 12 samples per sequencing run) |
Pooling, diluting, denaturing, ExAmp with cBot cluster generation (if required), & sequencing instrumentation set-up |
Internal
$55.52
Sample
|
|
| ► NGS library preparation (8) | |||
| Name | Description | Price | |
| 10X Genomics NextGEM Single Cell 3' (per sample) |
Using Trypan, the core will count cells and obtain viability of a single-cell suspension provided by the customer then proceed to library prep if sample(s) meet specifications. Pricing also includes Qubit and Fragment Analyzer trace analysis of the cDNA. |
Internal
$2,350.00
each
|
|
| DNA Exome |
43Mb capture size |
Inquire | |
| RRBS (per sample) |
Reduced-Representation Bisulfite Sequencing |
Internal
$202.25
each
|
|
| TruSeq Stranded mRNA (per sample) |
Dual-indexed libraries for analysis of high-quality mRNA |
Internal
$193.57
each
|
|
| TruSeq stranded total RNA (per sample) |
Dual-indexed libraries for analysis of high-quality total RNA to include coding and non-coding RNAs |
Internal
$272.12
Sample
|
|
| Takara SMARTer stranded total RNA (per sample) |
Dual-indexed libraries for analysis of small or degraded RNA |
Internal
$168.84
Sample
|
|
| NEBNext Ultra II DNA (per sample) |
Dual-indexed libraries for WGS |
Internal
$125.91
Sample
|
|
| QiaSeq DNA panel (per sample) |
Dual-indexed cancer-specific libraries with UMI |
Internal
$254.16
Sample
|
|
| ► NGS Informatics (1) | |||
| Name | Description | Price | |
| NGS Genomics Core Informatics (per sample) |
NGS pipeline analysis; Molecular Profiling Database processing & analysis |
Internal
$100.00
Sample
|
|
| ► Microarray (2) | |||
| Name | Description | Price | |
| MethylationEPIC - standard DNA (per sample) |
Genomie-wide analysis of >850K methylation sites in DNA from non-FFPE samples; must be in multiples of 8 |
Internal
$415.20
Sample
|
|
| MethylationEPIC - FFPE DNA (per sample) |
Genomie-wide analysis of >850K methylation sites in DNA from FFPE samples; must be in multiples of 8 |
Internal
$501.60
Sample
|
|
| ► Sequencing (4) | |||
| Name | Description | Price | |
| MiniSeq; 300 cycles mid-output (per flowcell) |
Paired-end sequencing with Illumina SBS chemistry |
Internal
$717.00
each
|
|
| NovaSeq S1 flow cell; 100 cycles |
1.3 - 1.6 billion clusters |
Inquire | |
| NovaSeq S1 flow cell; 200 cycles |
1.3 - 1.6 billion clusters |
Inquire | |
| NovaSeq S1 flow cell; 300 cycles |
1.3 - 1.6 billion clusters |
Inquire | |
| ► Consulting (1) | |||
| Name | Description | Price | |
| Custom projects for off-menu genomics services (per hour) |
Internal
$59.04
each
External $59.04 each |
||
| ► Droplet Digital PCR (1) | |||
| Name | Description | Price | |
| ddPCR |
The ddPCR service fee includes Droplet Reader Oil, a cartridge, a gasket, PCR plate, and foil seal to process 8 reactions. In addition to the sample(s), customers will supply appropriate Droplet Generation Oil, SuperMix, and assay(s). The Genomics Core will setup the reaction and instrumentation as well as assist in performing initial analysis in QuantaSoft. |
Internal
$51.87
set
|
|
