The Experimental Pathology core is experiencing a substantial backlog of service requests along with an increasing number of new requests. We are working hard to catch up, but it will take some time to do so. In the interim, we will need to prioritize services, and there may be significant delays in completing service requests. We appreciate your patience as we work through the backlog and return to normal operations. Feel free to reach out if you have any questions.
Overview of Services
The UAMS Experimental Pathology (ExPath) Core Laboratory provides researchers with centralized, comprehensive histological services. The laboratory can process and section tissues for use in biochemical, molecular, and pathologic studies. In addition, the laboratory works with investigators to develop immunostaining approaches to detect specific targets of interest and to identify clinical pathologists who can assist with image analysis and data interpretation. The laboratory director, consultants, and technicians have extensive experience in routine histology and immunohistochemistry involving human and animal tissues.
Services and Equipment
Tissue Processing and Embedding: The laboratory can process and paraffin embed tissues for downstream analysis. The lab is equipped to section frozen and paraffin-embedded tissues. Sections can be prepared for slides, molecular and biochemical studies, and laser capture dissection.
Tissue-Tek VIP 6 AI Tissue Processor and Tissue-Tek Embedding Center: For processing and paraffin embedding tissue
Microm HM325 Microtome: For sectioning paraffin-embedded tissues
Leica CM3050S Cryostat: For sectioning frozen tissue
Tissue Staining: The laboratory provides a variety of standard pathological stains for the histochemical analysis of tissues. In addition, the laboratory works with investigators to develop immunostaining approaches for detecting specific targets of interest.
Histo-Tek SL: Automated slide stainer for routine Hematoxylin and Eosin tissue staining
Leica Bond-Rx Fully Automated Research Stainer: Stainer that is capable of automated single and multiplexed immunostaining protocols.
Slide Scanning and Imaging: The laboratory is equipped with an Aperio digital slide scanner that can capture whole-slide images at 20x and 40x for subsequent analysis and archiving. In addition, the laboratory has an Akoya Mantra™ multispectral imaging system for capturing images with up to seven different colors on a single slide.
Leica (Aperio) Scanscope CS2: Has a five-slide capacity and can scan a whole slide at 20X or 40x magnification. Images are stored on a server where they can be shared and analyzed remotely.
Mantra™ Quantitative Pathology Workstation: Microscope equipped with a multispectral camera for multiplexed immunohistochemistry and imaging. inForm® Image Analysis software can spectrally unmix signals from 9 different fluorophores.
Consultation: Project planning is facilitated by consultation with investigators before project submission to the ExPath laboratory. ExPath staff can assist with antibody selection and optimization, protocol development, and troubleshooting. In addition, investigators can consult with clinical pathologists regarding data analysis and interpretation.
Leadership
Dr. Steven Post Laboratory Director 501-526-6046 SPost@uams.edu
Jennifer James Laboratory Manager 501-686-8265 JamesJenniferD@uams.edu
Location and hours of operation
Hours
Location
Monday - Friday from 8:00 am - 4:30 pm
4301 West Markham Street, Mail Slot 753 Little Rock, AR 72205 Lab located at WRCI-Walker Tower Bldg. Room 429
Welcome!
